kinase-glo® max reagent (Promega)
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kinase-glo® max reagent
Kinase Glo® Max Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kinase-glo%C2%AE+max+reagent/kinase+glo/pm40464690-82-26-30
Average 90 stars, based on 1 article reviews
Kinase Glo® Max Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kinase-glo%C2%AE+max+reagent/kinase+glo/pm40464690-82-26-30
Average 90 stars, based on 1 article reviews
kinase-glo® max reagent - by Bioz Stars,
2026-10
90/100 stars
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Inhibition:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of Activity Assay:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of Concentration Assay:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of Kinase Assay:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of Produced:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of In Vivo:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of Incubation:Article Title: The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition Article Snippet: Aliquots of 5 μL at various time points (2, 5, 10, 20 and 30 min) were transferred to a 384 well plate and mixed with 5 μL of Article Title: Novel thioxoimidazolidinone derivatives as dual EGFR and CDK2 inhibitors: Design, synthesis, anticancer evaluation with in silico study Article Snippet: Fifteen thioxoimidazolidinone derivatives were designed as dual EGFR and CDK2 inhibitors and synthesized following Claisen and Knoevenagel condensation.. Derivative 12 showed the best EGFR and CDK2 IC50 giving 0.098 μM and 0.087 μM, respectively, followed by 13 with IC50 0.108 μM and 0.144 μM, respectively.. Both derivatives showed better cancerous cell line growth inhibition than erlotinib over HCT-116, MCF-7, and HepG2. Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: The reactions containing 40 nM full-length ScIleRS or 100 nM Cterminal truncated ScIleRS, 200 μM ATP, 1 mM L-isoleucine, 1 mg/mL E. coli tRNAIle (produced in vivo), 30 mM HEPES pH 7.5, 150 mM NaCl, 30 mM KCl, 40 mM MgCl2, 1 mM DTT and 0.1% BSA were incubated in room temperature, and 10 μL time point aliquots were mixed with 10 μL of Article Title: Inhibitory mechanism of reveromycin A at the tRNA binding site of a class I synthetase Article Snippet: In the revised manuscript, we repeated ATP consumption assay with 200 μM Article Title: Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies. Article Snippet: Aim: This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches.. Methods and results: Fermentation of the isolated strain was done on fiv e media.. T he deriv ed e xtracts w ere in v estigated f or their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2), and hepatocellular carcinoma (HepG2) using MTT Assa y. T he fungal m y celia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7, and Caco-2 cell lines with IC 50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 μg mL −1 , respectiv ely. Article Title: NLRP12 downregulates the Wnt/ β -catenin pathway via interaction with STK38 to suppress colorectal cancer Article Snippet: HEK293T and HCT116 cells were stimulated with Wnt3a for 4h and lysed with RIPA buffer containing complete protease and phosphatase inhibitor cocktails (Roche). Article Title: The mechanism of lineage-specific tRNA recognition by bacterial tryptophanyl-tRNA synthetase and its implications for inhibitor discovery. Article Snippet: At various time points (2, 5, 10, 20, and 30 min), 5 μl aliquots were transferred to a 384-well plate and combined with 5 μl of |